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Inositol transport in mouse embryonic stem cells.

B D Higgins1, M T Kane

  • 1Department of Physiology, National University of Ireland, Galway.

Reproduction, Fertility, and Development
|November 3, 2005
PubMed
Summary
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Mouse embryonic stem cells utilize a specific transporter for myo-inositol uptake, which is sodium-dependent and influenced by cellular conditions. This suggests myo-inositol plays a role in osmoregulation for these cells.

Area of Science:

  • Cell Biology
  • Molecular Transport

Background:

  • Embryonic stem cells (ES cells) require precise nutrient regulation for development.
  • Myo-inositol is a crucial molecule involved in various cellular processes.

Purpose of the Study:

  • To characterize the mechanism of myo-inositol uptake in mouse ES cells.
  • To determine the kinetic parameters and regulatory factors of this transport process.

Main Methods:

  • Utilized radiolabeled [2-(3H]myo-inositol to quantify uptake in mouse ES cells.
  • Investigated the effects of sodium, temperature, time, and various inhibitors (glucose, phloridzin, ouabain) on uptake.
  • Assessed the influence of osmolality on myo-inositol transport.

Main Results:

Related Experiment Videos

  • Myo-inositol uptake is primarily a saturable, sodium-, time-, and temperature-dependent process.
  • Transport is distinct from glucose transporters and exhibits unidirectional characteristics (uptake > efflux).
  • Kinetic analysis revealed a Km of 65.1 ± 11.8 µmol/L and Vmax of 5.0 ± 0.59 pmol/µg protein/h.
  • Increased uptake in hypertonic conditions suggests myo-inositol acts as an osmolyte.

    • Conclusions:

    • Mouse ES cells possess a specific sodium-dependent myo-inositol transporter (SDMIT).
    • Myo-inositol transport is critical for ES cell function and potentially osmoregulation.