Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bioluminescence technology for imaging cell proliferation.

Hiroyuki Momota1, Eric C Holland

  • 1Department of Cancer Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Current Opinion in Biotechnology
|November 3, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Macrophage-secreted brain-derived neurotrophic factor promotes tumor growth in triple-negative breast cancer by inducing axonogenesis.

Cell death and differentiation·2026
Same author

CCN1/Cyr61 associates with β-amyloid levels in human cerebrospinal fluids.

iScience·2026
Same author

Therapeutic efficacy of dendritic cell vaccination in a novel syngeneic mouse model of diffuse hemispheric glioma, H3 G34-mutant.

Journal of neuro-oncology·2026
Same author

Development of PROTACs for targeted degradation of oncogenic TRK fusions.

RSC chemical biology·2026
Same author

Beyond Histology: A Unified Transcriptomic Atlas Defines Lung Cancer Biologic States and Subtypes.

bioRxiv : the preprint server for biology·2026
Same author

A systems approach identifies MERTK as a therapeutic vulnerability in ZFTA-RELA-driven ependymomas.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.

Current opinion in biotechnology·2026
Same journal

Medicinal plants fermentation: current knowledge and perspectives.

Current opinion in biotechnology·2026
Same journal

Fermented foods: lessons learned from metagenomics.

Current opinion in biotechnology·2026
Same journal

Microfluidic platforms for the transient transfection of mammalian cells: recent developments and challenges.

Current opinion in biotechnology·2026
Same journal

Harvesting insights from recent advances in yeast genomics for predictable and precision wine fermentation.

Current opinion in biotechnology·2026
Same journal

Minimal enzyme cascades for the aromatic-to-aromatic upgrading of lignin monomers.

Current opinion in biotechnology·2026
See all related articles

Monitoring cancer cell proliferation noninvasively is crucial. New transgenic mice use luciferase reporter genes to track tumor cell growth in real-time, aiding preclinical cancer research and therapy development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biotechnology

Background:

  • Cancer arises from uncontrolled cell proliferation due to genetic changes.
  • Monitoring tumor growth and cell proliferation is vital for understanding cancer development and treatment response.
  • Bioluminescence imaging (BLI) measures tumor cell number but not proliferation directly.

Purpose of the Study:

  • To develop a noninvasive method for monitoring cell proliferation in vivo.
  • To create reporter mice capable of visualizing tumor cell proliferation.
  • To enable real-time assessment of tumor cell proliferative activity.

Main Methods:

  • Developed transgenic mice expressing the luciferase gene under the E2F1 promoter.
  • Crossed reporter mice with genetically defined mouse cancer models.

Related Experiment Videos

  • Utilized bioluminescence imaging to detect light output proportional to cell proliferation.

    • Main Results:

    • Demonstrated that light production in reporter mice correlates with tumor cell proliferation.
    • Enabled noninvasive monitoring of proliferative activity in sporadic tumor models.
    • Showcased the potential for tracking biological pathways in living cells.

    Conclusions:

    • Transgenic mice with E2F1-driven luciferase reporters offer a novel tool for noninvasive monitoring of cancer cell proliferation.
    • This technology enhances preclinical cancer trials by providing real-time proliferation data.
    • The approach has broader applications for monitoring other biological processes in vivo.