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Updated: Aug 15, 2026

A Practical Guide to Phylogenetics for Nonexperts
Published on: February 5, 2014
mILD: a tool for constructing and analyzing matrices of pairwise phylogenetic character incongruence tests
Paul J Planet1, Indra Neil Sarkar
1Division of Invertebrate Zoology, American Museum of Natural History, New York, NY, USA.
Summary:
Pairwise comparisons of disagreement in phylogenetic datasets offer a powerful tool for isolating historical incongruence for closer analysis. Statistically significant phylogenetic character incongruence may reflect important differences in evolutionary history, such as horizontal gene transfer. Such testing can also be used to specify possible combinations of datasets for further phylogenetic analysis. The process of comparing multiple datasets can be very time consuming, and it is sometimes unclear how to combine data partitions given the observed patterns of incongruence. Here we present an application that automates the process of making pairwise comparisons between large numbers of phylogenetic datasets using the Incongruence Length Difference (ILD) test. The application also implements strategies for data combination based on the patterns of incongruence observed in pairwise comparisons.
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