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Updated: Aug 15, 2026

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Published on: August 11, 2018
Polar localization of a soluble methyl-accepting protein of Pseudomonas aeruginosa
Sonia L Bardy1, Janine R Maddock
1Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, 48109, USA.
Abstract:
A soluble methyl-accepting chemotaxis protein (MCP) of Pseudomonas aeruginosa, McpS, showed polar localization by immunofluorescence microscopy. Overexpression of McpS resulted in a dominant-negative effect on chemotaxis and caused a loss of polar clustering of the general MCP population. The polar localization of a soluble MCP defines a third, and unexpected, paradigm for cellular MCP localization.
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