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Updated: Aug 3, 2026
11:12
Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat
Published on: February 8, 2011
A mathematical model of the euglycemic hyperinsulinemic clamp
Umberto Picchini1, Andrea De Gaetano, Simona Panunzi
1CNR-IASI BioMatLab, Rome, Italy. umberto.picchini@biomatematica.it
Theoretical Biology & Medical Modelling
|November 5, 2005
Summary
Prolonging the Euglycemic Hyperinsulinemic Clamp (EHC) beyond two hours in obese individuals reveals continued increases in glucose uptake. Mathematical modeling of EHC data enhances understanding of insulin resistance components in obesity.
Area of Science:
- Metabolic Research
- Physiology
- Endocrinology
Background:
- The Euglycemic Hyperinsulinemic Clamp (EHC) is the gold standard for measuring insulin sensitivity.
- Standard EHC protocols typically last two hours.
- Obesity is associated with altered insulin sensitivity.
Purpose of the Study:
- To investigate the effects of prolonged EHC (five hours) on insulin sensitivity in subjects with varying BMIs.
- To analyze insulin resistance components in obese versus lean individuals using a mathematical model.
Main Methods:
- Performed a five-hour Euglycemic Hyperinsulinemic Clamp (EHC) on sixteen subjects (BMI 18.5-63.6 kg/m²).
- Applied a mathematical model incorporating delays to EHC data.
- Assessed hepatic glucose output, pancreatic insulin secretion, and tissue insulin resistance.
Main Results:
- Glucose uptake rates continued to rise in obese subjects when the EHC procedure was extended beyond two hours.
- Obese subjects exhibited less effective suppression of hepatic glucose output and higher insulin secretion compared to lean subjects.
- While tissue insulin resistance appeared higher in obese individuals, this did not reach statistical significance.
Conclusions:
- Extending EHC duration provides further insights into glucose uptake dynamics in obese individuals.
- Mathematical modeling of EHC data allows for a more comprehensive analysis of insulin resistance.
- The study highlights differences in metabolic regulation between obese and lean subjects.
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