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Updated: Aug 15, 2026

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
Published on: April 28, 2022
Hadamard frequency-encoded SOFAST-HMQC for ultrafast two-dimensional protein NMR
Paul Schanda1, Bernhard Brutscher
1Institut de Biologie Structurale, Jean-Pierre Ebel C.N.R.S.-C.E.A.-UJF, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France.
Abstract:
We demonstrate the feasibility of recording (1)H-(15)N correlation spectra of proteins in only one second of acquisition time. The experiment combines recently proposed SOFAST-HMQC with Hadamard-type (15)N frequency encoding. This allows site-resolved real-time NMR studies of kinetic processes in proteins with an increased time resolution. The sensitivity of the experiment is sufficient to be applicable to a wide range of molecular systems available at millimolar concentration on a high magnetic field spectrometer.
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