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Updated: Aug 15, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
Published on: December 23, 2022
Diversity of "Candidatus Liberibacter asiaticus," based on the omp gene sequence
C Bastianel1, M Garnier-Semancik, J Renaudin
1Laboratoire de Biologie Cellulaire et Moléculaire, UMR GDPP, INRA, Université Victor Segalen Bordeaux 2, 71 avenue Edouard Bourlaux BP81, 33883 Villenave D'Ornon Cedex, France.
Abstract:
Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, "Candidatus Liberibacter africanus" in Africa and "Candidatus Liberibacter asiaticus" in Asia. In this study, we used an omp-based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of "Ca. Liberibacter asiaticus" isolates. By using five different enzymes, each the 10 isolates tested could be associated with a specific combination of restriction profiles. The results indicate that the species "Ca. Liberibacter asiaticus," even within a given region, may comprise several different variants. Thus, omp-based PCR-RFLP analysis is a simple method for detecting and differentiating "Ca. Liberibacter asiaticus" isolates.
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