Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rat breathalyzer.

Martin A Javors1, Brett C Ginsburg, Greg Friesenhahn

  • 1Department of Psychiatry, University of Texas Health Science Center, San Antonio, Texas 78229, USA. javors@uthscsa.edu

Alcoholism, Clinical and Experimental Research
|November 5, 2005
PubMed
Summary

A novel rat breathalyzer accurately estimates blood alcohol concentration (BAC) using breath alcohol concentration (BrAC) measurements. This noninvasive method provides identical pharmacokinetic profiles, enabling convenient, repeated alcohol level assessments in laboratory settings.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Brain Structural Abnormalities in Addiction Disorders: Transdiagnostic Meta-Analyses.

Human brain mapping·2026
Same author

Examining Phosphatidylethanol (PEth) Formation Rates Within and Between Subjects in Ex Vivo Whole Blood.

Drug testing and analysis·2026
Same author

Spatial memory in Alzheimer's disease 5XFAD mice is enhanced by XPO1 inhibitor KPT-330.

GeroScience·2026
Same author

Canagliflozin Reprograms the Aging Hippocampus in Genetically Diverse UM-HET3 Mice and Attenuates Alzheimer's-Like Pathology.

Aging cell·2025
Same author

Canagliflozin reprograms the aging hippocampus in genetically diverse UM-HET3 mice and attenuates Alzheimer's-like pathology.

bioRxiv : the preprint server for biology·2025
Same author

Correction to: Lifespan effects in male UM‑HET3 mice treated with sodium thiosulfate, 16-hydroxyestradiol, and late‑start canagliflozin.

GeroScience·2025

Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Animal Models

Background:

  • Accurate estimation of blood alcohol concentration (BAC) is crucial in toxicological and pharmacological research.
  • Current methods for BAC measurement can be invasive and require frequent sampling.
  • Development of noninvasive techniques for monitoring alcohol levels is highly desirable.

Purpose of the Study:

  • To characterize the estimation of blood alcohol concentration (BAC) in laboratory rats.
  • To validate a specialized apparatus for measuring breath alcohol concentration (BrAC).
  • To establish a correlation between BrAC and BAC for noninvasive alcohol level assessment.

Main Methods:

  • Utilized a specialized apparatus comprising a body chamber and head chamber for breath sample collection from rats.

Related Experiment Videos

  • Employed gas chromatography with a flame ionization detector for quantifying ethanol in breath samples.
  • Collected simultaneous blood and breath samples from catheterized Lewis rats for comparative analysis.

    • Main Results:

    • Lewis rats demonstrated comfort and trainability within the apparatus for extended periods (up to 150 min).
    • Pharmacokinetic profiles of breath alcohol concentration (BrAC) and blood alcohol concentration (BAC) were virtually identical.
    • A consistent conversion factor (3282) was established between BrAC (peak area) and BAC (mM), allowing direct BAC estimation from BrAC.

    Conclusions:

    • The developed rat breathalyzer offers an accurate and convenient noninvasive laboratory method for estimating BAC.
    • This technique is particularly valuable for studies necessitating repeated alcohol level monitoring.
    • The study validates BrAC measurement as a reliable surrogate for BAC in preclinical research.