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Function of c-Fos-like and c-Jun-like proteins on trichostatin A-induced G2/M arrest in Physarum polycephalum

Xiao-Xue Li1, Jun Lu, Yan-Mei Zhao

  • 1Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.

Acta Biochimica Et Biophysica Sinica
|November 5, 2005
PubMed

Insights

In Physarum polycephalum, c-Fos-like and c-Jun-like proteins regulate cell cycle progression. These proteins are crucial for G2/M transition and histone acetylation, impacting cell division.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Transcription factors c-Fos and c-Jun homologs are present in the slime mold Physarum polycephalum.
  • These proteins are observed during the synchronous cell cycle progression in P. polycephalum.

Purpose of the Study:

  • To investigate the role of c-Fos-like and c-Jun-like proteins in the G2/M transition of the cell cycle in P. polycephalum.
  • To determine the involvement of these proteins in regulating Cyclin B1 levels and histone acetylation during cell cycle control.

Main Methods:

  • Antibody neutralization was used to interrupt the functions of c-Fos-like and c-Jun-like proteins.
  • The effect of histone deacetylase inhibitor trichostatin A on G2/M transition and protein levels was examined.
  • Changes in protein levels and acetylation status of c-Fos-like and c-Jun-like proteins were analyzed.

Main Results:

  • Interruption of c-Fos-like and c-Jun-like protein functions led to arrest at the G2/M transition phase.
  • These proteins regulate the level of Cyclin B1, a key factor in cell cycle progression.
  • Histone deacetylase inhibition caused alterations in c-Fos-like and c-Jun-like protein levels and hyperacetylation of c-Jun-like protein.

Conclusions:

  • c-Fos-like and c-Jun-like proteins are essential for regulating G2/M transition in P. polycephalum.
  • These proteins are implicated in histone acetylation-dependent cell cycle control.
  • Coordinated expression and hyperacetylation of c-Fos-like and c-Jun-like proteins are involved in regulating the G2/M transition.

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