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Related Experiment Videos

Circulating endothelial cells in acute ischaemic stroke.

Sunil K Nadar1, Gregory Y H Lip, Kaeng W Lee

  • 1Haemostasis Thrombosis and Vascular Biology Unit, University Department of Medicine, City Hospital, Birmingham, UK.

Thrombosis and Haemostasis
|November 8, 2005
PubMed
Summary

Acute ischemic stroke significantly increases circulating endothelial cells (CECs), markers of endothelial damage. These CECs correlate with established markers of endothelial dysfunction, confirming vasculopathy in stroke patients.

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Area of Science:

  • Vascular Biology
  • Neurology
  • Biomarkers

Background:

  • Endothelial cell damage is implicated in acute ischemic stroke.
  • CD146-bearing circulating endothelial cells (CECs) are direct indicators of endothelial damage.
  • Elevated CECs may correlate with other markers of endothelial dysfunction like von Willebrand factor (vWf) and soluble E-selectin.

Purpose of the Study:

  • To investigate if CECs are elevated in acute ischemic stroke patients.
  • To determine the correlation between CECs and plasma vWf and soluble E-selectin levels.
  • To compare CEC levels across acute stroke, high-risk hypertension, and normotensive control groups.

Main Methods:

  • Studied 29 acute ischemic stroke patients, 30 high-risk hypertensive patients, and 30 normotensive controls.

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  • Quantified CECs using CD146 immunobead capture.
  • Measured plasma vWf and soluble E-selectin via ELISA.

    • Main Results:

    • Acute ischemic stroke patients exhibited significantly higher CECs, plasma vWf, and soluble E-selectin compared to control groups.
    • CECs showed significant positive correlations with soluble E-selectin (r=0.432, p<0.001) and vWf (r=0.349, p=0.001).
    • Multivariate analysis indicated that the clinical group (stroke, hypertension, or healthy) was the primary determinant of CEC levels.

    Conclusions:

    • Acute ischemic stroke is associated with a significant increase in circulating endothelial cells (CECs).
    • Elevated CECs in stroke patients correlate with established plasma markers of endothelial dysfunction, confirming severe vasculopathy.
    • Clinical status is the most significant factor influencing CEC numbers.