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Updated: Aug 3, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat
Huw D Jones1, Angela Doherty, Huixia Wu
1CPI Division, Rothamsted Research, Harpenden, AL5 2JQ, UK. huw.jones@bbsrc.ac.uk
Abstract:
Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic lines in both spring and winter wheat varieties.

