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Related Experiment Videos

Hypercholesterolemia and apolipoprotein B expression: regulation by selenium status.

Sanjiv Dhingra1, Mohinder P Bansal

  • 1Department of Biophysics, Panjab University, Chandigarh-160014, India. sanjivdhingra@gmail.com

Lipids in Health and Disease
|November 8, 2005
PubMed
Summary

Selenium deficiency increases apolipoprotein B (apoB) in hypercholesterolemia, while selenium supplementation downregulates apoB. This highlights selenium

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Area of Science:

  • Nutritional biochemistry
  • Lipid metabolism research
  • Animal model studies

Background:

  • Apolipoprotein B (apoB) facilitates LDL clearance via LDL receptors.
  • Emerging evidence links selenium (Se) to lipid metabolism regulation.
  • Experimental hypercholesterolemia models are crucial for studying lipid disorders.

Purpose of the Study:

  • To investigate the impact of selenium deficiency and supplementation on apoB expression.
  • To elucidate the role of selenium in managing hypercholesterolemia-induced apoB changes.
  • To assess hepatic LDL receptor activity under varying selenium conditions.

Main Methods:

  • Utilized male Sprague Dawley rats fed high-cholesterol diets.
  • Administered varying levels of selenium (deficiency and 1 ppm supplementation).

Related Experiment Videos

  • Quantified apoB levels (ELISA), protein expression (Western blot), and LDL receptor mRNA (RT-PCR).

    • Main Results:

    • Selenium deficiency with high cholesterol diet significantly increased apoB and decreased LDL-R after 2 months.
    • 1 ppm selenium supplementation significantly decreased apoB and increased LDL-R after 2 months.
    • One month of treatment showed no significant changes in apoB or LDL-R.

    Conclusions:

    • Selenium deficiency upregulates apoB expression during experimental hypercholesterolemia.
    • Selenium supplementation (1 ppm) downregulates apoB expression.
    • Results underscore the nutritional importance of selenium in lipid metabolism.