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Related Experiment Videos

Variable tRNA content in HIV-1IIIB.

M Jiang1, J Mak, M A Wainberg

  • 1Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.

Biochemical and Biophysical Research Communications
|June 30, 1992
PubMed
Summary
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This study analyzed low molecular weight RNA in HIV-1, specifically transfer RNA (tRNA). Results show the number of tRNA species varies by cell type, with fewer than four essential for the viral life cycle.

Area of Science:

  • Virology
  • Molecular Biology
  • RNA Biochemistry

Background:

  • Human Immunodeficiency Virus type 1 (HIV-1) contains various low molecular weight RNAs.
  • Transfer RNA (tRNA) plays a crucial role in viral replication, including acting as a primer for reverse transcriptase.

Purpose of the Study:

  • To characterize the tRNA species present in HIV-1 produced in different cell types.
  • To determine the presence and association of the primer tRNA, tRNA(Lys,3), within HIV-1.

Main Methods:

  • Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) to analyze viral tRNA.
  • Hybridization with a tRNA(Lys,3)-specific DNA probe.
  • Comparison of electrophoretic mobility with purified human tRNA(Lys,3).

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Main Results:

  • The number of tRNA species in infectious HIV-1 varied from over 20 to 4, depending on the cell type.
  • 1-3 tRNA species were tightly associated with viral genomic RNA.
  • tRNA(Lys,3) was detected in HIV-1 from H9 cells but not from HeLa cells, with differing electrophoretic mobilities.

Conclusions:

  • Fewer than four tRNA species appear necessary for the HIV-1 infectious life cycle.
  • The presence and association of tRNA(Lys,3) are cell-type dependent.
  • Cellular environment influences the tRNA composition of HIV-1.