Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

UNC-13 interaction with syntaxin is required for synaptic transmission.

Jon M Madison1, Stephen Nurrish, Joshua M Kaplan

  • 1Department of Molecular Biology, Massachusetts General Hospital, Simches Research Building, 185 Cambridge Street, Boston, Massachusetts 02114, USA.

Current Biology : CB
|November 8, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Updates in the Diagnosis and Management of Hypertension: A Review of Recent Guidelines.

British journal of hospital medicine (London, England : 2005)·2026
Same author

Neuron-intrinsic and glial pathways regulate sensory cilia regeneration in adult <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2026
Same author

Activity induced GEM-4/Copine expression inhibits gap junctions and promotes thermosensory plasticity.

bioRxiv : the preprint server for biology·2026
Same author

Transsynaptic linking of calcium channels and postsynaptic receptors at a dyadic synapse.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

An enteric neuron ionotropic receptor regulates salt stress resistance.

Nature·2026
Same author

A lateralized sensory signaling pathway mediates context-dependent olfactory plasticity in <i>Caenorhabditis elegans</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026

The UNC-13 protein

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Neurotransmitter secretion is crucial for synaptic function.
  • UNC-13 protein is known to be involved in vesicle priming at synapses.
  • Existing models suggest UNC-13 stabilizes Syntaxin's open conformation.

Purpose of the Study:

  • To investigate the functional significance of the UNC-13/Syntaxin interaction in vivo.
  • To determine the role of UNC-13 in synaptic vesicle priming versus fusion.

Main Methods:

  • Utilized a truncated UNC-13 protein in C. elegans models.
  • Introduced double mutations (F1000A/K1002A) to disrupt UNC-13/Syntaxin interaction.
  • Measured endogenous synaptic event rates and excitatory post-synaptic currents (EPSCs).

Related Experiment Videos

Main Results:

  • A truncated UNC-13 protein partially rescued unc-13 mutant defects.
  • Disrupting the UNC-13/Syntaxin interaction reduced synaptic event rates and EPSC amplitudes.
  • The pool of primed vesicles remained unaffected by the mutation.

Conclusions:

  • The UNC-13/Syntaxin interaction is conserved in C. elegans.
  • This interaction is essential for nerve-evoked vesicle fusion, not priming.
  • UNC-13 may regulate multiple stages of the synaptic vesicle cycle.