Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Catch-bond model derived from allostery explains force-activated bacterial adhesion.

Wendy Thomas1, Manu Forero, Olga Yakovenko

  • 1Department of Bioengineering, University of Washington, Seattle, Washington, USA. wendyt@u.washington.edu

Biophysical Journal
|November 8, 2005
PubMed
Summary

High shear enhances Escherichia coli adhesion via FimH, suggesting catch bonds. We found two distinct states, indicating FimH forms allosteric catch bonds regulated by mechanical force.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenotype-Genotype Discordance in Antimicrobial Resistance of <i>Acinetobacter baumannii</i>: Implications for Diagnostics and Surveillance.

Pathogens (Basel, Switzerland)·2026
Same author

Mechanomedicine.

Nature reviews bioengineering·2026
Same author

A hybrid PKPD agent-based model of the tumour immune interaction: effects of anti-cancer combination therapy.

Journal of pharmacokinetics and pharmacodynamics·2026
Same author

Early Mycobacterial Antigens in the Immunodiagnosis of Latent Tuberculosis Infection.

Pathogens (Basel, Switzerland)·2026
Same author

Regulation of platelet contractility by agonists present across a thrombus.

Blood advances·2026
Same author

rFVIIIa-platelet binding enhances platelet procoagulant activity independently of thrombin generation.

Blood vessels, thrombosis & hemostasis·2026

Area of Science:

  • Microbiology
  • Biophysics
  • Biochemistry

Background:

  • High shear forces enhance Escherichia coli adhesion to mannose-coated surfaces through the FimH adhesin.
  • This phenomenon raises questions about the nature of FimH-mediated bonds, specifically whether they exhibit catch bond behavior.

Purpose of the Study:

  • To investigate the duration of pauses for E. coli on mannosylated surfaces.
  • To determine if FimH forms allosteric catch bonds regulated by mechanical force.

Main Methods:

  • Analysis of pause duration data for E. coli on mannosylated surfaces.
  • Development of a mathematical model based on chemical allostery to describe catch bond lifetime.
  • Estimation of model parameters to correlate with FimH structural changes.

Related Experiment Videos

Main Results:

  • Observed a double exponential decay in pause duration, indicating two distinct conformational states.
  • The mathematical model successfully explained the observed data, including the double exponential decay and force-dependent binding.
  • Model parameters aligned with known force-induced structural changes in FimH.

Conclusions:

  • E. coli FimH likely forms allosteric catch bonds.
  • Mechanical force regulates transitions between conformational states with different unbinding rates.
  • The study advances understanding of catch bonds and allostery in protein regulation.