Related Experiment Video
Updated: Aug 15, 2026

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
Published on: June 23, 2018
Prevention of thermal induced aggregation of cytochrome c at isoelectric pH values by polyanions
Diana Fedunová1, Marián Antalík
1Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, Kosice.
Abstract:
Differential scanning calorimetry, viscometry, optical and CD spectroscopy were used to characterize the influence of two polyanions, poly(vinylsulfate) (PVS), and poly(4-styrene-sulfonate) (PSS) on thermal transition reversibility of ferricytochrome c at or near isoelectric pH. In these conditions, both PVS and PSS enhance the thermal transition reversibility of cytochrome c by preventing the aggregation of denatured protein molecules. Data indicate that the polyanions are in complex with cytochrome c that is stabilized by synergistic effect of Coulombic and non-Coulombic interactions.
Related Concept Videos
Colloidal precipitates
Coagulation
The Colloidal State

