Related Experiment Video
Updated: Aug 15, 2026
Growing Desmoplastic Three-Dimensional Pancreatic Cancer Spheroids from Co-Culture
Published on: September 27, 2024
[Metallothionein and its isoform genes expression in the human pancreatic cancer cell strains and their function]
Yong Xie1, Yu-pei Zhao, Ge Chen
1Department of General Surgery, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Objective:
To compare the expression of metallothionein (MT) genes and proteins in six human pancreatic cancer cell strains and two human pancreatic cancer drug-resistant cell strains and to explore the relationship between the expression of the MT and pancreatic cancer cell chemo-resistance.
Methods:
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to determine the MT isoform-specific mRNA, and cadmium/hemoglobin saturation-electrochemistry to determine MT protein levels.
Results:
MT protein expression in the pancreatic cancer cell strains was encoded by MT-1A, MT-1B, MT-1E, MT-1F, MT-1G, MT-1X, and MT-2A genes. The expression of MT proteins was upregulated and MT-1B, MT-1E, MT-1X, MT-2A genes overexpressed in human pancreatic cancer drug-resistant cell lines (P < 0.05).
Conclusion:
Expressions of MT proteins and genes correlate with the proliferation and chemoresistance of human pancreatic cancer cell strains.
