Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plant protoplasts as genetic tool: selectable markers for developmental studies.

I Negrutiu1, S Hinnisdaels, D Cammaerts

  • 1Laboratory of Plant Genetics, Vrije Universiteit Brussel, Belgium.

The International Journal of Developmental Biology
|March 1, 1992
PubMed
Summary

Protoplast-derived selectable markers offer powerful insights into plant genetics. These tools aid in studying amino acid pathways, alien gene introgression via protoplast fusion, and foreign gene stability in transgenic plants.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reformed islets: a long-term primary cell platform for exploring mouse and human islet biology.

Cell death discovery·2024
Same author

Comparison of imputation methods for missing production data of dairy cattle.

Animal : an international journal of animal bioscience·2023
Same author

Review: When worlds collide - poultry modeling in the 'Big Data' era.

Animal : an international journal of animal bioscience·2023
Same author

Interferon-β Decreases the Hypermetabolic State of Red Blood Cells from Patients with Multiple Sclerosis.

ACS chemical neuroscience·2022
Same author

Outcome of older hip fracture patients on anticoagulation: a comparison of vitamin K-antagonists and Factor Xa inhibitors.

Archives of orthopaedic and trauma surgery·2020
Same author

Sequential Time-Kill, a Simple Experimental Trick To Discriminate between Pharmacokinetics/Pharmacodynamics Models with Distinct Heterogeneous Subpopulations versus Homogenous Population with Adaptive Resistance.

Antimicrobial agents and chemotherapy·2020

Area of Science:

  • Plant Biology
  • Genetics
  • Molecular Biology

Background:

  • Protoplasts are versatile tools in plant biology.
  • Their application in studying genetic systems is expanding.
  • Selectable markers derived from protoplasts enhance genetic analysis.

Purpose of the Study:

  • To demonstrate the utility of protoplast-derived selectable markers.
  • To investigate disturbed genetic systems affecting plant development.
  • To explore applications in amino acid biosynthesis, alien gene introgression, and gene stability.

Main Methods:

  • Utilizing protoplast-derived selectable markers.
  • Studying auxotrophic mutants for amino acid pathways.
  • Employing protoplast fusion for alien genetic introgression.

Related Experiment Videos

  • Analyzing foreign gene expression stability in transgenic plants across generations.

    • Main Results:

    • Protoplast markers facilitate the study of amino acid biosynthesis and transport.
    • Protoplast fusion enables introgression of alien genetic information.
    • Foreign gene expression stability in transgenic plants can be assessed across meiotic generations.

    Conclusions:

    • Protoplast-derived selectable markers are effective for dissecting complex genetic systems.
    • These methods advance understanding of plant development and genetic modification.
    • Protoplast technology offers novel approaches for plant genetic research.