Glucose biosensor based on Au nanoparticles-conductive polyaniline nanocomposite

Area of Science:

• Nanomaterials Science
• Electrochemistry
• Biosensors

Background:

• Development of sensitive and selective glucose biosensors is crucial for diabetes management.
• Existing biosensors face challenges in stability and detection limits.
• Nanocomposite materials offer unique properties for enhanced biosensor performance.

Purpose of the Study:

• To develop a novel glucose biosensor utilizing a composite of gold nanoparticles (Au NPs) and conductive polyaniline (PANI) nanofibers.
• To immobilize glucose oxidase (GOx) and Nafion onto the nanocomposite surface for electrochemical detection.
• To evaluate the analytical performance, reproducibility, and stability of the developed glucose biosensor.

Main Methods:

• Fabrication of Au NPs-PANI nanofibers nanocomposite.
• Immobilization of glucose oxidase (GOx) and Nafion onto the nanocomposite.
• Electrochemical detection of hydrogen peroxide (H2O2) generated from glucose oxidation.
• Linear sweep voltammetry and chronoamperometry for performance evaluation.

Main Results:

• The glucose biosensor demonstrated a linear response over a wide concentration range (1.0x10(-6) to 8.0x10(-4) mol/L).
• A high sensitivity with a slope of 2.3 mA/M and a low detection limit of 5.0x10(-7) M (S/N=3) was achieved.
• Excellent reproducibility (R.S.D. < 5%) and good operational stability (over 2 weeks) were observed.

Conclusions:

• The Au NPs-PANI nanofibers nanocomposite provides a promising platform for developing highly sensitive and selective glucose biosensors.
• The developed electrochemical biosensor exhibits excellent analytical performance and stability, suitable for practical applications.
• This work highlights the potential of nanomaterial composites in advancing biosensor technology for healthcare.