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Published on: October 30, 2013

Preparation and characterization of yeast and human desumoylating enzymes

Shyr-Jiann Li1, William Hankey, Mark Hochstrasser

  • 1Celera, South San Francisco, CA 94020, USA.

Methods in Enzymology
|November 9, 2005
PubMed
Abstract

No abstract available in PubMed .

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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...