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Related Experiment Videos

Distinct immunosuppressive effect by Isodon serra extracts.

Yan Zhang1, Jianwen Liu, Wei Jia

  • 1State Key Laboratory of Bioreactor Engineering, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, PR China.

International Immunopharmacology
|November 9, 2005
PubMed
Summary

Four ent-Kaurene Diterpenoids from Isodon serra suppress lymphocyte overproduction. Enmein, the most potent compound, inhibits DNA replication and reduces inflammation and Interleukin-2 in vivo.

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Area of Science:

  • Immunology
  • Pharmacology
  • Natural Products Chemistry

Background:

  • Abnormal lymphocyte proliferation contributes to autoimmune diseases.
  • Isodon serra is a traditional herb with potential medicinal properties.

Purpose of the Study:

  • To investigate the immunosuppressive effects of ent-Kaurene Diterpenoids from Isodon serra.
  • To identify the most potent compound and elucidate its mechanism of action.

Main Methods:

  • MTT assay and Flow Cytometry Analyses (FCAS) for in vitro studies.
  • ELISA assay and mouse tumescence model for in vivo studies.

Main Results:

  • Four ent-Kaurene Diterpenoids suppressed Concanavalin A-stimulated murine splenic T lymphocytes.

Related Experiment Videos

  • Enmein exhibited the strongest inhibitory effect (IC50/EC50 = 1.55) by interfering with G1-S DNA replication and cell cycle regulation.
  • Enmein reduced ear swelling and Interleukin-2 levels in a dose-dependent manner in vivo.

    • Conclusions:

    • Ent-Kaurene Diterpenoids from I. serra possess distinct in vitro and in vivo immunosuppressive properties.
    • Enmein demonstrates significant potential as an immunosuppressive agent.
    • Further research is needed to fully elucidate the profound mechanisms of Enmein.