Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA splicing capability of live neuronal dendrites.

J Glanzer1, K Y Miyashiro, J-Y Sul

  • 1Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 9, 2005
PubMed
Summary

Dendrites possess the machinery for precursor-messenger RNA (pre-mRNA) splicing. This study confirms that dendrites can perform pre-mRNA splicing, indicating a novel cellular function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025
Same author

Improving cosmological reach of a gravitational wave observatory using Deep Loop Shaping.

Science (New York, N.Y.)·2025
Same author

Squeezing the quantum noise of a gravitational-wave detector below the standard quantum limit.

Science (New York, N.Y.)·2024
Same author

Multifaceted roles of CCAR family proteins in the DNA damage response and cancer.

Experimental & molecular medicine·2024
Same author

Search for Subsolar-Mass Binaries in the First Half of Advanced LIGO's and Advanced Virgo's Third Observing Run.

Physical review letters·2022

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Dendrites are crucial neuronal extensions involved in RNA and protein metabolism.
  • While some RNA-binding proteins are found in dendrites, their pre-mRNA splicing capacity is largely unknown.

Purpose of the Study:

  • To investigate the presence and functionality of pre-mRNA splicing components within intact dendrites.
  • To determine if dendrites possess the capability to perform pre-mRNA splicing.

Main Methods:

  • Transfection of isolated dendrites with reporter pre-mRNAs (chicken delta-crystallin and luciferase).
  • In vitro synaptoneurosome experiments to assess splicing factor activity.
  • Analysis of splicing junctions in transfected dendrites and synaptoneurosome fractions.

Related Experiment Videos

Main Results:

  • Demonstrated the presence and functionality of pre-mRNA splicing components in dendrites.
  • Observed splicing junctions at expected sites in reporter pre-mRNAs within isolated dendrites.
  • Confirmed splicing activity in synaptoneurosome fractions using chicken delta-crystallin pre-mRNA.

Conclusions:

  • Dendrites contain functional pre-mRNA splicing machinery.
  • Dendroplasm harbors splicing factors capable of promoting pre-mRNA splicing.
  • This suggests a previously unrecognized role for dendrites in RNA processing.