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Microsatellite polymorphism in Japanese mongrel dogs.
Noboru Oishi1, Mari Maeda, Koichi Makimura
1Laboratory of Biophysics, School of Medicine, Teikyo University, Tokyo, Japan.
The Journal of Veterinary Medical Science
|November 9, 2005
Summary
Genetic variability in Japanese dogs was assessed using ten microsatellite loci. These markers demonstrated high discriminatory power, proving effective for canine genetic testing and kinship analysis in Japan.
Area of Science:
- Canine genetics
- Population genetics
- Forensic genetics
Background:
- Understanding genetic diversity is crucial for conservation and management of dog populations.
- Mongrel dogs in Japan represent a diverse genetic pool across various regions.
Purpose of the Study:
- To evaluate the genetic variability of mongrel dogs across Japan.
- To assess the utility of ten microsatellite loci for canine genetic identification and kinship testing.
Main Methods:
- Blood samples were collected from 182 unrelated mongrel dogs nationwide.
- Ten microsatellite loci were analyzed across autosomal chromosomes.
- Hardy-Weinberg equilibrium and polymorphic information content (PIC) were calculated.
Main Results:
- All ten microsatellite loci were in Hardy-Weinberg equilibrium.
- High polymorphic information content (PIC) values ranged from 0.58 to 0.80.
- High power of discrimination (PD) values ranged from 0.80 to 0.94.
- A combined mean exclusion chance (MEC) of 0.9995 was achieved.
Conclusions:
- The selected microsatellite loci exhibit significant genetic variability in Japanese mongrel dogs.
- These markers are highly effective for individual identification and kinship testing in this population.
- The findings support the application of these genetic markers in forensic and population studies of Japanese dogs.