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Expression and purification of human angiotensinogen in Chinese hamster ovary cells
T Hatae1, E Takimoto, A Fukamizu
1Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Biochimica Et Biophysica Acta
|June 24, 1992
Abstract:
We have produced human angiotensinogen in Chinese hamster ovary (CHO) cells. The expression products were purified to homogeneity by a single column chromatography and its 17 amino-terminal sequences were identical to those of the native protein. We demonstrated the recombinant human angiotensinogen to be a substrate for human renin.