Related Experiment Video
Updated: Aug 15, 2026
Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography
Published on: January 7, 2015
A histologic study of adverse effects of different cosmetic skin fillers
Meire B Parada1, Nílceo S Michalany, Karime M Hassun
1Department of Dermatology, Federal University of São Paulo, São Paulo, Brazil. mbparada@ip2.com.br
Objective:
This study was designed to describe the histologic aspects of the cutaneous and mucosal adverse reactions to fillers used for cosmetic purposes.
Methods And Materials:
Eleven specimens of adverse reactions caused by Restylane, Artecoll, Metacril, New-Fill, Aquamid, and liquid silicone were processed and stained by hematoxylin-eosin and Alcian blue and submitted to polarized light.
Results:
Most of the reactions were foreign-body granulomas.
Conclusions:
The type of granuloma and the characteristics of the agent were determined, contributing to the histologic identification of the material used. In the cases involving Restylane and Aquamid, it was possible to identify the material using Alcian blue stain and by the presence of necrosis. This stain was strongly positive in Restylane and faintly positive in Aquamid, and intense necrosis was observed in Aquamid cases. In the other cases the filler material itself was recognized and identified.
