CD36 involvement in orosensory detection of dietary lipids, spontaneous fat preference, and digestive secretions
- 1Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation et Centre Européen des Sciences du Goût, UMR 5170 CNRS/1214 INRA/Université de Bourgogne, Dijon, France.
- 0Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation et Centre Européen des Sciences du Goût, UMR 5170 CNRS/1214 INRA/Université de Bourgogne, Dijon, France.
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View abstract on PubMed
Summary
This summary is machine-generated.Rats and mice are attracted to fats, suggesting an oral fat detection system. The study found that the fatty acid transporter CD36 is crucial for detecting long-chain fatty acids (LCFAs) in the mouth.
Area Of Science
- Neuroscience
- Physiology
- Molecular Biology
Background
- Rodents show a natural preference for lipids, indicating a potential orosensory system for dietary fat detection.
- The fatty acid transporter CD36 has a high affinity for long-chain fatty acids (LCFAs) and is present in rat lingual papillae, making it a candidate for oral fat sensing.
Purpose Of The Study
- To investigate the role of the fatty acid transporter CD36 in the orosensory detection of long-chain fatty acids (LCFAs).
- To determine if CD36 is responsible for the preference for LCFAs in rodents.
Main Methods
- RT-PCR to analyze gene expression of lipid-binding proteins in mouse lingual and palatal papillae.
- Immunostaining to localize CD36 protein in taste buds.
- Behavioral studies in wild-type and CD36-null mice to assess preference for LCFA-enriched solutions and diets.
- Physiological measurements in rats and wild-type mice with esophageal ligation to evaluate pancreatobiliary secretions in response to oral LCFAs.
Main Results
- CD36 expression was found exclusively in lingual papillae, not palatal papillae, in mice.
- CD36 protein localized to the apical side of circumvallate taste bud cells.
- Mice lacking CD36 (CD36-null) showed no preference for LCFA-enriched solutions or diets.
- Oral administration of unsaturated LCFAs induced a rapid and sustained increase in pancreatobiliary secretions in rats and wild-type mice.
Conclusions
- CD36 plays a significant role in the oral detection of long-chain fatty acids (LCFAs).
- The findings suggest that alterations in lingual fat perception mediated by CD36 may be associated with feeding dysregulation.
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