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Published on: June 25, 2020
[Detection and identification of phytoplasma strain associated with periwinkle yellows]
Hong Cai1, Baohua Kong, Hairu Chen
1Key Laboratory for Plant Pathology of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China.
Abstract:
A 1.2kb and 1.3kb DNA fragments were amplified in DNA samples extracted from periwinkle exhibiting yellows naturally. The amplified fragments were cloned, sequenced and compared with other phytoplasma strains. The results showed that the 16S rDNA and ribosomal protein gene of periwinkle yellows phytoplasma consist of 1239bp and 1244bp, respectively. They all shared high homology (higher than 97.5%) with phytoplasma strains of 16S rI group. Thus, the result made it clear that the phytoplasma strain of periwinkle yellows (PY) is one of the members of phytoplasma 16SrI group.
Insights
Periwinkle yellows phytoplasma DNA was sequenced, revealing 16S rDNA and ribosomal protein genes. This phytoplasma strain belongs to the 16SrI group, sharing high homology with related phytoplasma strains.
Area of Science:
- Molecular Biology
- Plant Pathology
- Phytoplasmology
Context:
- Periwinkle plants naturally exhibiting yellowing symptoms were investigated.
- Phytoplasmas are plant-pathogenic bacteria lacking cell walls, known to cause significant agricultural losses.
- Accurate identification and classification of phytoplasma strains are crucial for disease management.
Purpose:
- To molecularly characterize the phytoplasma strain associated with periwinkle yellows.
- To determine the phylogenetic relationship of the periwinkle yellows phytoplasma to other known phytoplasma groups.
Summary:
- DNA fragments (1.2kb and 1.3kb) were amplified from infected periwinkle samples.
- The amplified 16S rDNA (1239bp) and ribosomal protein gene (1244bp) were sequenced.
- High homology (>97.5%) was found with strains in the 16SrI group, confirming the periwinkle yellows phytoplasma (PY) belongs to this group.
Impact:
- Provides definitive molecular identification of the periwinkle yellows phytoplasma.
- Contributes to the understanding of phytoplasma diversity and classification within the 16SrI group.
- Facilitates targeted disease control strategies for periwinkle and potentially other crops affected by related phytoplasmas.
