[Chitosanase production in convoluted fibrous bed bioreactor with two-step process]

Chitosanase activity can be increased remarkably by a two-step process, in which, the growth of T. reesei was in the first step with glucose as carbon source and enzyme induction by D-glucosamine in the second step. With the novel process chitosanase activity produced was about 100% higher than that in the traditional one step process. Under the optimized culture conditions: aeration rate was 4L/min (2 vvm) and age of mycelia were 72 h, The activity of chitosanase produced by immobilized T. reesei in convoluted fibrous bed bioreactor(CFBB) was 246mU/mL which was about 39% higher than that with free cell culture in 5L CSTR under the same culture conditions. Furthermore, the novel bioreactor was also feasible to be operated in repeated batch mode. During 10 repeated-batch in 15 days, the average production of chitosanase activity to each batch was kept 235mU/mL.