[Study on submerged culture conditions of lipase inhibitor producing strain]
Related Experiment Video
Updated: Aug 15, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
1College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China. wh-zhong@sina.com
Abstract:
The optimum medium and fermentations conditions of the lipase inhibitor producing strain Bacillus sp. LF were studied. The optimal medium was selected after single factor test and orthogonal test as follows: lactose 0.8%, peptone 1%, (NH4)2SO4 0.8%, NaCl 0.5%, original pH 7.2. With 4% (V/V) inoculum in 40 mL medium in 250 mL flask, Bacillus sp. LF could reach high yield of lipase inhibitor after 24 h at 30 degrees C. The highest lipase inhibitory activity in broth could reach 52.3%.
More Related Videos
10:17Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
Published on: October 9, 2016
08:29Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies
Published on: January 19, 2016