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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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1St George's Hospital, London, UK. jball@sgul.ac.uk
Critical Care (London, England)
|November 10, 2005
Summary
Controversial practices in blood transfusion, albumin use, lipoproteins in sepsis, and pulmonary artery catheterization persist. Emerging nitric oxide therapy offers a potential advancement despite ongoing medical errors.
Area of Science:
- Critical care medicine
- Cardiovascular research
- Sepsis management
Background:
- Current medical practices in critical care face significant controversies.
- Areas of debate include blood transfusion protocols, albumin administration, lipoprotein management in sepsis, and the use of pulmonary artery catheters.
Purpose of the Study:
- To highlight persistent controversies in critical care.
- To acknowledge the prevalence of medical errors.
- To introduce potential new therapeutic avenues.
Main Methods:
- Review of existing literature and clinical practice guidelines.
- Analysis of common points of contention in critical care.
Main Results:
- Significant controversies identified in blood transfusion, albumin, lipoproteins in sepsis, and pulmonary artery catheter use.
- High rates of medical errors noted in current practice.
Conclusions:
- Ongoing debate necessitates further research and guideline refinement.
- Emerging therapies, such as nitric oxide, may offer future improvements in patient care.
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