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A barley cDNA clone encoding a type III chlorophyll a/b-binding polypeptide of the light-harvesting complex II
J Brandt1, V S Nielsen, H Thordal-Christensen
1Department of Plant Biology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
Abstract:
The nucleotide sequence of a leaf cDNA clone encoding a Type III chlorophyll a/b-binding (CAB) protein of light-harvesting complex II (LHCII) in barley is reported. Sequence comparisons and results from in vitro import into chloroplasts demonstrate that the cDNA clone encodes a functional transit peptide of 45 amino acid residues and a mature polypeptide of 223 residues with a predicted molecular mass of 24.3 kDa. After insertion into thylakoids, the mature protein is resistant to protease attack. Hybridization analysis using a gene-specific probe shows that the gene is expressed in dark-grown seedlings and that the amount of mRNA increases during illumination.