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[Cold storage of platelet suspension by adding trehalose]
Cheng-Yin Huang1, Rong-Cai Tang, Li Cai
1Department of Transfusion Blood, Jiangsu Province Blood Center, Nanjing 210042, China. Huangchengyin1223@126.com
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|November 10, 2005
Summary
Trehalose effectively preserves rabbit platelets during cold storage, maintaining their function and extending circulation time. This method offers a promising approach for enhancing platelet viability and transfusion efficacy.
Area of Science:
- Biomedical Science
- Hematology
- Biochemistry
Context:
- Platelet storage and transfusion are critical in medical treatments.
- Current methods for platelet preservation have limitations in maintaining long-term viability and function.
- Cold storage of platelets is challenging due to cold-induced activation and damage.
Purpose:
- To investigate the efficacy of trehalose as a cryoprotective agent for storing rabbit platelets at low temperatures.
- To evaluate the impact of trehalose treatment on platelet function and survival after cold storage.
- To determine the optimal concentration of trehalose for preserving platelet viability.
Summary:
- Rabbit platelet concentrates treated with 50 mg/ml trehalose were stored at 4-8°C.
- (51)Cr-labeling and in vitro platelet aggregometry assessed platelet survival and function.
- Trehalose-treated platelets showed preserved function and extended circulation survival compared to untreated controls.
Impact:
- Trehalose significantly protects chilled rabbit platelets, extending their circulation time.
- This method maintains platelet function, crucial for transfusion efficacy.
- The findings suggest trehalose is a viable agent for improving platelet storage and transfusion protocols.