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Updated: Jul 10, 2026

Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
Published on: May 9, 2020
[Relationship between key enzyme activities of inosine-producing pathway and inosine accumulation]
Yongbo Song1, Xianpeng Cai, Ju Chu
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. songyongbo@sina.com
Abstract:
The specific activities of key enzymes relating to the pathway of inosine synthesis of three different bacterial strains including high-yield, low-yield and wild strains were determined and compared systematically. A close relationship between inosine production and the specific activities of key enzymes was found. According to the enzyme characteristics of high-yield strain, suggestions on further strain improvement by modification of genetic engineering were proposed. Enzymology study is believed to be an effective way to make screening of high-yield strains more efficient.
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