[Study on morphological change of aspergillus flavus damaged by citral with a multi-channel micro-spectrophotometer...

Citral extracted from litsea cubeb oil was used as antibacterial drug; toxigenic and atoxigenic Aspergillus flavus cell (AFC) were used as its target. Multi-channel micro-spectrophotometer (MMSP) and micro-image analysis were applied. Under the pressure of citral, changes in AFC were measured, including items such as absorbance, area, perimeter, major axis length and minor axis length. We found that toxigenic AFC had two characteristic absorbent peaks at 410 nm and 665 nm, and that the waveforms of absorption spectrum of toxigenic and atoxigenic AFC migrated and their peak areas increased, and that four morphological parameters reduced with citral concentration increasing. The results suggest that citral damages selective permeability and structure of membrane to enter to cell, and acts on target molecule or organelle to bring changes in physiology and biochemistry. In real-time, in-situ and non-invasive situation, the dynamic changes in shape and intracellular macromolecule are fastly measured during the drugs damage a living cell, which not only provides these changes with necessary physical parameters, but also has an importance in theoretical research and experimental method.