Increased transient receptor potential channel TRPC3 expression in spontaneously hypertensive rats
Daoyan Liu1, Alexandra Scholze, Zhiming Zhu
1Medizinische Klinik IV, Charité Campus Benjamin Franklin, Berlin, Germany.
Background:
Disturbances in the regulation of cytosolic calcium concentration have been attributed to primary hypertension, but the role of calcium-permeable transient receptor potential canonical channel 3 (TRPC3) has not yet been evaluated in primary hypertension.
Methods:
Expression of TRPC3 was determined using in-cell Western assay. Evaluation of RNA interference for the downregulation of a specific gene in cells by small interfering RNA was performed. Measurements of cytosolic calcium were carried out using the fluorescent dye fura2.
Results:
Expression of TRPC3 was significantly increased in monocytes from spontaneously hypertensive rats (SHR) compared with normotensive Wistar-Kyoto rats (WKY). Transplasmamembrane calcium influx and thapsigargin-induced sustained calcium increase were significantly higher in SHR compared with WKY. In the presence of the TRP channel blocker SKF-96365 these differences were no longer observed. Specific TRPC3-knockdown by transfection of monocytes from SHR with small interfering RNA significantly reduced TRPC3 expression, trans-plasma membrane calcium influx, and thapsigargin-induced sustained calcium increase.
Conclusions:
This study shows, for the first time, increased TRPC3 channel expression and increased TRPC3-related calcium influx in SHR.
