Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

An oligodendrocyte precursor cell line from rat optic nerve.

G Almazan1, R McKay

  • 1Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge 02139.

Brain Research
|May 8, 1992
PubMed
Summary

Researchers created permanent rat optic nerve cell lines using a temperature-sensitive oncogene. These neural precursor cells can differentiate into oligodendrocytes at specific temperatures, offering a new model for studying myelin development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Renal cell carcinoma: ESMO Clinical Practice Guideline for diagnosis, treatment and follow-up.

Annals of oncology : official journal of the European Society for Medical Oncology·2024
Same author

Resistance to COVID-19 vaccination has increased in Ireland and the United Kingdom during the pandemic.

Public health·2021
Same author

Injury to the Soft Tissues by Dentures.

The Dental register·2021
Same author

Investigating Tongue Strength and Endurance in Children Aged 6 to 11 Years.

Dysphagia·2019
Same author

Impact of symptomatic skeletal events on health-care resource utilization and quality of life among patients with castration-resistant prostate cancer and bone metastases.

Prostate cancer and prostatic diseases·2017
Same author

The defensive function of persecutory delusions: an investigation using the Implicit Association Test.

Acta neuropsychiatrica·2016

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Establishing stable neural precursor cell lines is crucial for studying cell differentiation and development.
  • Conditional immortalization techniques offer a way to control cell proliferation and differentiation.

Purpose of the Study:

  • To establish permanent rat optic nerve cell lines using a temperature-sensitive oncogene.
  • To investigate the differentiation potential of these cell lines into oligodendrocyte precursors.

Main Methods:

  • Primary rat optic nerve cultures were infected with a replication-deficient retrovirus carrying a temperature-sensitive Simian Virus 40 large T-antigen (tsA58 and U19 mutations).
  • Infected cells were selected using the antibiotic G418, and a clonal cell line (tsU19-5) was established.

Related Experiment Videos

  • Cellular properties and marker expression were assessed at permissive (33°C) and non-permissive (39°C) temperatures.

    • Main Results:

    • The tsU19-5 cell line proliferated and expressed oligodendrocyte precursor markers (A2B5, vimentin, CNP) at the permissive temperature (33°C).
    • At the non-permissive temperature (39°C), cells showed potential for further differentiation, expressing oligodendrocyte-specific markers including galactocerebroside, myelin basic protein, and proteolipid protein.
    • The temperature-sensitive oncogene allowed for conditional control over proliferation and differentiation.

    Conclusions:

    • Conditional oncogenes can be used to establish neural precursor cell lines.
    • These cell lines retain the capacity for in vitro differentiation into specific neural cell types, such as oligodendrocytes.
    • This approach provides a valuable tool for studying neural development and differentiation processes.