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Related Experiment Videos

Enumerating leptospires using the coulter counter.

Christina M Humberd1, Clinton K Murray, Saundra K Stuart

  • 1Infectious Diseases Department, Wilford Hall USAF Medical Center, San Antonio, Texas 78236, USA. Christina.Humberd@lackland.af.mil

The American Journal of Tropical Medicine and Hygiene
|November 12, 2005
PubMed
Summary
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Counting leptospires, or bacteria, using a Coulter counter is a faster and easier alternative to the traditional Petroff-Hausser counting chamber method. This technique provides reliable and reproducible results for bacterial enumeration.

Area of Science:

  • Microbiology
  • Bacteriology
  • Medical Technology

Background:

  • The standard method for counting leptospires involves Petroff-Hausser counting chambers and dark-field microscopy.
  • This technique is effective but requires significant technical skill and time.

Purpose of the Study:

  • To evaluate the Coulter counter as a potentially faster and less demanding alternative for quantifying leptospires.
  • To compare the accuracy and reliability of Coulter counter measurements against the established gold standard.

Main Methods:

  • Leptospires were enumerated using both a Coulter counter and a Petroff-Hausser counting chamber with dark-field microscopy.
  • Counts obtained from both methods were compared for reliability and reproducibility.

Main Results:

Related Experiment Videos

  • The Coulter counter provided counts that were comparable to those obtained using the Petroff-Hausser counting chamber.
  • The Coulter counter method proved to be significantly faster and less technically demanding.

Conclusions:

  • The Coulter counter offers a viable and efficient alternative for the enumeration of leptospires.
  • This method may replace traditional techniques, improving laboratory workflow and accessibility for bacterial quantification.