Related Experiment Video
Updated: Aug 14, 2026
Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay
Published on: March 12, 2018
Fas expression in lung metastasis from osteosarcoma patients
Nancy Gordon1, Carola A S Arndt, Douglas S Hawkins
1Division of Pediatrics, Department of Cancer Biology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, and Division of Pediatrics Hematology/Oncology, Mayo Clinic, Rochester, MN, USA.
Osteosarcoma (OS) lung metastases often lack Fas expression, allowing tumor cells to evade immune detection. Chemotherapy may reverse this by increasing Fas expression in OS cells.
Area of Science:
- Oncology
- Immunology
Background:
- Osteosarcoma (OS) metastasis is a significant challenge in patient outcomes.
- Animal studies suggest Fas expression inversely correlates with OS metastatic potential.
Purpose of the Study:
- To investigate Fas expression status in human OS lung metastases.
- To explore the relationship between Fas expression and chemotherapy in OS metastasis.
Main Methods:
- Immunohistochemistry was used to analyze Fas expression in 38 patient lung metastasis specimens.
- Correlation analysis was performed between Fas expression and preoperative chemotherapy administration.
Main Results:
- 60% of OS lung metastases analyzed were Fas negative.
- Weakly positive Fas expression was observed in 32% of samples.
- A significant correlation (P = 0.0013) was found between Fas expression and preoperative salvage chemotherapy.
Conclusions:
- Loss of Fas expression may be a mechanism for OS cells to evade host immune surveillance in the lungs.
- Preoperative chemotherapy might induce OS tumor regression by upregulating Fas expression.
More Related Videos
11:15A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
Published on: May 6, 2018
08:47Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells
Published on: August 21, 2012