Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oligonucleotide structure influences the interactions between cationic polymers and oligonucleotides.

Sumati Sundaram1, Sandra Viriyayuthakorn, Charles M Roth

  • 1Department of Chemical & Biochemical Engineering, Rutgers University, 98 Brett Road, Piscataway, New Jersey 08854, USA.

Biomacromolecules
|November 15, 2005
PubMed
Summary

Oligodeoxynucleotide (ODN) structure significantly impacts how they bind to cationic polymers like poly-L-lysine (pLL). Hairpin ODNs bind more readily and release slower than unstructured ODNs, influencing delivery strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aerosol Delivery of Polyelectrolyte Surfactant-Antimicrobial Nanoparticles to the Lungs.

Pharmaceutical research·2026
Same author

Antimicrobial Loaded Graft-Copolymer Nanoparticles for Treatment of <i>Pseudomonas aeruginosa</i> Infections.

bioRxiv : the preprint server for biology·2025
Same author

Simulating Interclonal Interactions in Diffuse Large B-Cell Lymphoma.

Bioengineering (Basel, Switzerland)·2023
Same author

Pharmacodynamic Model of the Dynamic Response of <i>Pseudomonas aeruginosa</i> Biofilms to Antibacterial Treatments.

Biomedicines·2023
Same author

Biomechanics of regenerated esophageal tissue following the implantation of a tissue engineered Cellspan<sup>TM</sup> Esophageal Implant.

Journal of biomechanics·2022
Same author

Esophageal regeneration following surgical implantation of a tissue engineered esophageal implant in a pediatric model.

NPJ Regenerative medicine·2022

Area of Science:

  • Biochemistry
  • Materials Science
  • Polymer Chemistry

Background:

  • Cationic polymers are crucial for delivering nucleic acids like oligodeoxynucleotides (ODNs).
  • Understanding polymer-ODN interactions is key for optimizing delivery systems.
  • ODN structure is a potential factor influencing complex formation and stability.

Purpose of the Study:

  • To investigate how ODN secondary structure affects complex formation with cationic polymers.
  • To compare the binding affinity and dissociation kinetics of unstructured vs. hairpin structured ODNs with poly-L-lysine (pLL).
  • To assess the implications of these interactions for ODN delivery applications.

Main Methods:

  • Formation of complexes between poly-L-lysine (pLL) and both unstructured and hairpin structured ODNs.

Related Experiment Videos

  • Quantification of complex formation using dye exclusion assays at varying pLL:ODN charge ratios.
  • Assessment of complex dissociation using heparin challenge assays.
  • Determination of ODN release kinetics and dose-response curves.

    • Main Results:

    • Hairpin structured ODNs formed complexes with pLL at lower charge ratios compared to unstructured ODNs.
    • Higher fractions of hairpin ODNs were complexed at high charge ratios.
    • Unstructured ODNs showed greater absolute amounts and faster kinetic rates of release upon heparin challenge.
    • ODN structure significantly influences the association and dissociation dynamics of pLL-ODN complexes.

    Conclusions:

    • ODN secondary structure plays a critical role in the stability and dissociation of cationic polymer-ODN complexes.
    • These findings are vital for selecting appropriate ODN sequences for therapeutic delivery.
    • The design of polymeric carriers for efficient cellular ODN delivery can be informed by these structural insights.