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Related Experiment Videos

Exercise training induces respiratory substrate-specific decrease in Ca2+-induced permeability transition pore

Mariannick Marcil1, Karine Bourduas, Alexis Ascah

  • 1Départment de Kinesiology, Université de Montréal, PO Box 6128 Centre-Ville, Montreal, PQ, Canada H3C 3J7.

American Journal of Physiology. Heart and Circulatory Physiology
|November 15, 2005
PubMed
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Regular exercise training reduces the sensitivity of heart mitochondria to calcium-induced permeability transition pore opening, particularly when using complex II substrates. This finding highlights exercise

Area of Science:

  • Mitochondrial physiology
  • Cardiovascular research
  • Exercise science

Background:

  • Mitochondria play a crucial role in cellular energy production and cell death.
  • The permeability transition pore (PTP) in mitochondria is implicated in various pathologies.
  • Exercise is known to induce beneficial adaptations in the heart.

Purpose of the Study:

  • To investigate the effect of regular exercise on mitochondrial susceptibility to calcium-induced PTP opening.
  • To explore if exercise alters the modulation of PTP opening by physiological effectors.
  • To determine substrate-specific regulation of PTP opening in trained rat hearts.

Main Methods:

  • Isolated rat heart mitochondria were subjected to treadmill running for 10 weeks.
  • Fluorimetric assays were used to measure Ca(2+)-induced PTP opening.

Related Experiment Videos

  • Respiration, membrane potential, reactive oxygen species, and Ca(2+) uptake were assessed.

    • Main Results:

    • Exercise training did not affect basal or ADP-stimulated mitochondrial respiration.
    • A higher Ca(2+) load was required to induce PTP opening with complex II substrates compared to complex I substrates.
    • Training significantly increased the Ca(2+) threshold for PTP opening with complex II substrates, independent of cyclosporin A.

    Conclusions:

    • Heart mitochondria exhibit substrate-specific regulation of the PTP.
    • Regular exercise training confers a reduced sensitivity of heart mitochondria to Ca(2+)-induced PTP opening, especially with complex II substrates.
    • These adaptations may contribute to the cardioprotective effects of exercise training.