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Related Experiment Videos

Renal cell adaptation to oxalate.

Eddie L Greene1, Gerard Farell, Shihui Yu

  • 1Division of Nephrology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.

Urological Research
|November 15, 2005
PubMed
Summary

Chronic hyperoxaluria can harm kidneys. Renal cells adapt to oxalate exposure by increasing HSP-70, suggesting in vivo adaptive mechanisms against kidney damage.

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Area of Science:

  • Nephrology
  • Cell Biology
  • Biochemistry

Background:

  • Chronic hyperoxaluria leads to kidney stones, scarring, and function loss.
  • Oxalate exposure in cultured renal cells causes cell death and proliferation.

Purpose of the Study:

  • To investigate the time course and cell-type specificity of renal cell responses to oxalate.
  • To explore adaptive mechanisms in renal cells exposed to oxalate.

Main Methods:

  • Cultured proximal and distal renal epithelial cells and interstitial fibroblasts were exposed to oxalate (0.5-2.0 mM).
  • Reactive oxygen species (ROS) generation was measured using DCF.
  • Cell number was quantified using CyQuant; HSP-70 expression was assessed via real-time PCR and Western blot.

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Main Results:

  • Oxalate exposure induced ROS generation in all tested renal cell types.
  • Initially, cells showed death and decreased numbers, but adapted and grew by 48-72 hours.
  • This adaptation correlated with increased HSP-70 mRNA and protein expression.

Conclusions:

  • Renal cells exhibit adaptive responses to oxalate exposure, including ROS generation and subsequent growth.
  • Increased HSP-70 expression appears to be a key component of this adaptive mechanism.
  • In vivo renal cells may possess mechanisms to tolerate chronic hyperoxaluria via chaperone molecule induction.