Related Experiment Video
Updated: Aug 14, 2026
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
Published on: May 10, 2019
[Expression of Runx2/Cbfa1 in the developing pulpo-dentinal complex of postnatal mice]
Ke-qing Pan1, Shu Li, Pi-shan Yang
1Dept. of Periodontology, School of Stomatology, Shandong University, Jinan 250012, China.
Objective:
To study the expression of Runx2/Cbfa1 in the developing dentin and differentiating odontoblasts.
Methods:
A postnatal mice teeth developing model was built histologically. Immunohistochemical technique was adopted to determine the expression of Runx2/Cbfa1 in the developing pulpo-dentinal complex in mice.
Results:
Runx2/Cbfa1 was merely present in predentin in the exact and before the 11th day's postnatal stages. Meanwhile, it was positively located in odontoblasts and dental pulp cells in root region, but negatively in coral part after the 11th day's stages.
Conclusion:
Runx2/Cbfa1 may play an important role in the deposing of tooth dentin and in the differentiating of odontoblasts and pulp cells.
