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Related Experiment Video

Updated: Jul 2, 2026

Measuring Replicative Life Span in the Budding Yeast
12:41

Measuring Replicative Life Span in the Budding Yeast

Published on: June 25, 2009

Sir2 blocks extreme life-span extension.

Paola Fabrizio1, Cristina Gattazzo, Luisa Battistella

  • 1Andrus Gerontology Center and Department of Biological Sciences, University of Southern California, Los Angeles, California 90089, USA.

Cell
|November 16, 2005
PubMed
Summary
This summary is machine-generated.

Inactivating Sir2 (silent information regulator 2) extends yeast chronological life span, opposite to its effect on replicative life span. This involves ethanol metabolism and stress gene upregulation.

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Last Updated: Jul 2, 2026

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A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae
10:39

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Area of Science:

  • Molecular Biology
  • Genetics
  • Aging Research

Background:

  • Sir2 (silent information regulator 2) is a conserved deacetylase influencing lifespan and stress response across species.
  • In yeast, Sir2 is crucial for maintaining replicative lifespan, with increased dosage delaying aging.

Purpose of the Study:

  • To investigate the role of Sir2 in regulating chronological lifespan in yeast.
  • To understand the molecular mechanisms underlying Sir2's influence on yeast aging.

Main Methods:

  • Genetic manipulation, including deletion of the SIR2 gene (sir2Δ).
  • Analysis of chronological lifespan under various conditions, including calorie restriction and mutations in Sch9 or Ras pathways.
  • Measurement of ethanol uptake/catabolism and gene expression profiling (stress-resistance and sporulation genes).

Main Results:

  • Loss of Sir2 function significantly extends yeast chronological lifespan, an effect opposite to its role in replicative lifespan.
  • Sir2 inactivation leads to increased ethanol consumption and upregulation of stress-resistance and sporulation genes.
  • Extending the lifespan of sir2Δ mutants requires severe calorie restriction or additional genetic mutations.

Conclusions:

  • The impact of SIR2 on yeast lifespan is context-dependent, with opposing effects on replicative versus chronological aging.
  • Sir2's role in aging is complex and may involve intricate regulation of metabolic and stress-response pathways.
  • Findings suggest that Sir2-like deacetylases may have complex, potentially opposing, roles in aging processes in higher eukaryotes.