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Updated: Aug 14, 2026
Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing (Frog) Embryo
Published on: April 21, 2022
Proteomic analysis of the Gallus gallus embryo at stage-29 of development
David Agudo1, David Agudo Garcillán, Francisco Gómez-Esquer
1Departamento de Ciencias de la Salud III, Facultad de Ciencias de la Salud, Universidad Rey Juan Carlos, Avenida de Atenas, S/N E28922 Alcorcón, Madrid, Spain. david.agudo@urjc.es
Abstract:
The chicken (Gallus gallus) is one of the primary models for embryological and developmental studies. In order to begin to understand the molecular mechanisms underlying the normal and abnormal development of the chicken, we used 2-DE to construct a whole-embryo proteome map. Proteins were separated by IEF on IPG strips, and by 11% SDS-PAGE) gels. Protein identification was performed by means of PMF with MALDI-TOF-MS. In all, 105 protein spots were identified, 35 of them implicated in embryo development, 10 related with some diseases, and 16, finally, being proteins that have never been identified, purified or characterized in the chicken before. This map will be updated continuously and will serve as a reference database for investigators, studying changes at the protein level under different physiological conditions.
