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Characterization of two small cryptic plasmids from Pseudomonas sp. strain S-47
Jong-Chan Chae1, Chi-Kyung Kim, Gerben J Zylstra
1Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, NJ 08901, USA.
Biochemical and Biophysical Research Communications
|November 18, 2005
Summary
Two cryptic plasmids, p47L and p47S, from Pseudomonas sp. S-47 were analyzed. Plasmid p47L belongs to a known rolling-circle replicating (RCR) plasmid family, while p47S represents a novel RCR plasmid family.
Area of Science:
- Molecular Biology
- Microbial Genetics
- Plasmid Biology
Background:
- Pseudomonas sp. S-47 harbors two small cryptic plasmids, p47L and p47S.
- Understanding plasmid replication mechanisms is crucial for microbial genetics and biotechnology.
Purpose of the Study:
- To characterize the DNA sequences, physical, and functional maps of plasmids p47L and p47S.
- To determine the replication mechanism and classify these plasmids within known families.
Main Methods:
- DNA sequencing and physical mapping of plasmids p47L and p47S.
- Analysis of single-strand DNA formation to infer replication mechanism.
- Sequence comparison of the replication initiation (rep) gene and homology searches.
Main Results:
- Plasmids p47L (3084 bp) and p47S (1782 bp) were fully sequenced, exhibiting high GC content.
- Detection of single-strand DNA indicated a rolling-circle replication (RCR) mechanism for both plasmids.
- Plasmid p47L's rep gene showed homology to the pIJ101/pJV1 RCR plasmid family; p47S's rep gene defined a new RCR plasmid family.
Conclusions:
- Plasmids p47L and p47S utilize the rolling-circle replication mechanism.
- Plasmid p47L is classified into an existing RCR plasmid family.
- Plasmid p47S represents a novel RCR plasmid family, expanding the known diversity of bacterial plasmids.