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Updated: Aug 14, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
Classification and comparison of capillary columns by determination of the solution enthalpy of polar and non polar
S Vezzani1, P Moretti, G Castello
1University of Genova, Dipartimento di Chimica e Chimica Industriale, Via Dodecaneso 31, I-16146 Genova, Italy.
Abstract:
The behaviour and separation characteristics of capillary columns containing different stationary phases (bonded methyl- and methylphenylsiloxanes and polyglycols, and carbon layer and porous polymer) were classified and compared by measuring the values of the solution enthalpy DeltaHs of n-alkanes and 1-alcohols at various temperatures. The difference in DeltaHs values between straight-chain 1-alcohols and n-alkanes with the same number of carbon atom (DeltaDeltaHs) does not change with changing temperature when gas-liquid partition stationary phases are used, but depends on the column composition and polarity and therefore permits to evaluate the influence of polarity on the solute-solvent interaction. The trend of the DeltaDeltaHs values is correlated with that of the DeltaC parameter and with the McReynolds' polarity constants.
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