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A colorimetric method for detection and quantification of chlorinating activity of hemeperoxidases

Kelath Murali Manoj1, Lowell P Hager

  • 1Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. kmm@mail.wsu.edu

Analytical Biochemistry
|November 18, 2005
PubMed

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Precipitation Titration: Endpoint Detection Methods01:19

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In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...