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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
'Click chemistry' on polysaccharides: a convenient, general, and monitorable approach to develop
Teruaki Hasegawa1, Mariko Umeda, Munenori Numata
1Department of Chemistry and Biotechnology, Graduate School of Engineering, Kyushu University, Hakozaki, 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan.
Abstract:
(1-->3)-beta-D-Glucans having various functional appendages (lactoside, ferrocene, pyrene, and porphyrin) can be prepared in an convenient, quantitative, and regioselective manner through regioselective bromination-azidation of curdlan to afford 6-azido-6-deoxycurdlan followed by chemoselective [3+2]-cycloadditions with various functional modules bearing a terminal alkyne group. The ability to monitor reaction conversions is an additional advantage of this synthetic approach over the conventional direct modifications on polysaccharides; the reaction can be readily monitored based on the intensity of azido peaks in the in situ attenuated total reflection infrared spectra.
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