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Updated: Aug 10, 2026
An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
Published on: April 17, 2013
Expression of TAp73 and DeltaNp73 isoform transcripts in thyroid tumours
1Laboratoire d'Oncologie Moléculaire EA3805, PBS, Cité Hospitalière de la Milétrie, Avenue du Recteur Pineau, 86021 Poitiers Cedex, France.
Aim:
This study was aimed to determine p73 status in thyroid tumours.
Methods:
Differential expression of the TAp73, DeltaTAp73 transcripts was measured in a panel of 60 thyroid malignancies by quantitative RT-PCR.
Results:
By comparison to normal thyroid tissue surrounding the tumours, we observed significant downregulation of TP73 transcripts in adenomas and in differentiated carcinomas. Correlations were found in normal tissue specimens between the expression of TAp73 and DeltaNp73 transcripts and that of p53, p14ARF p16INK4a, but these correlations were lost in carcinomas (PTC or FTC).
Conclusions:
We have found significant variations of TAp73, DeltaNp73, p53, p14ARF p16INK4a, expressions and correlations between the expressions of those different genes in thyroid cancer.
Insights
p73 gene expression is significantly reduced in thyroid tumors, including adenomas and differentiated carcinomas, compared to normal tissue. These changes disrupt normal correlations with other tumor suppressor genes like p53.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- The p73 gene plays a crucial role in tumor suppression.
- Understanding p73 expression patterns in thyroid malignancies is vital for cancer research.
Purpose of the Study:
- To investigate the expression status of p73 transcripts (TAp73 and DeltaNp73) in various thyroid tumors.
- To analyze the correlation between p73 expression and other key genes (p53, p14ARF, p16INK4a) in thyroid cancer.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was employed to measure transcript levels.
- The study analyzed a panel of 60 thyroid malignancies and compared them to adjacent normal thyroid tissue.
Main Results:
- Significant downregulation of TP73 transcripts was observed in thyroid adenomas and differentiated carcinomas relative to normal thyroid tissue.
- In normal thyroid tissue, TAp73 and DeltaNp73 expression correlated with p53, p14ARF, and p16INK4a.
- These correlations were notably absent in papillary (PTC) and follicular (FTC) carcinomas.
Conclusions:
- Thyroid tumors exhibit significant alterations in TAp73 and DeltaNp73 expression.
- The study highlights disrupted correlations between p73, p53, p14ARF, and p16INK4a in thyroid cancer, suggesting a role in tumorigenesis.
