Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vesicular roundness and compound release in PC-12 cells.

D Germain1, D Maysinger, M I Glavinovic

  • 1Department of Computer Engineering, McGill University, Montreal, Canada.

Journal of Neuroscience Methods
|November 18, 2005
PubMed
Summary

This study quantitatively analyzed dense core vesicles in PC-12 cells. Homotypic fusion was found to be not prominent, even after chronic treatment to enhance release.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selected mitochondrial DNA landscapes activate the SIRT3 axis of the UPR<sup>mt</sup> to promote metastasis.

Oncogene·2017
Same author

Super-resolution microscopy reveals a golden kiss of death to mitochondria.

Cell death discovery·2016
Same author

Involvement of the BCL2 Gene in 131 Cases of Non-Hodgkin's B Lymphomas: Analysis of Correlations with Immunological Findings and Cell Cycle.

Leukemia & lymphoma·2016
Same author

Sirtuins and the Estrogen Receptor as Regulators of the Mammalian Mitochondrial UPR in Cancer and Aging.

Advances in cancer research·2016
Same author

Celastrol targets proteostasis and acts synergistically with a heat-shock protein 90 inhibitor to kill human glioblastoma cells.

Cell death & disease·2014
Same author

The inner ear of Megatherium and the evolution of the vestibular system in sloths.

Journal of anatomy·2013

Area of Science:

  • Cell Biology
  • Neuroscience
  • Biophysics

Background:

  • Dense core vesicles (DCVs) are crucial for regulated secretion in neuroendocrine cells.
  • Understanding DCV dynamics, including fusion, is key to deciphering cellular communication.
  • PC-12 cells are a standard model for studying neurosecretion.

Purpose of the Study:

  • To develop a quantitative morphological analysis of DCV spatial and regional properties.
  • To assess the prominence of homotypic fusion in PC-12 cells under basal and stimulated conditions.
  • To investigate the impact of chronic treatment on DCV morphology and fusion.

Main Methods:

  • Computerized image processing of electron micrographs to obtain binary images of DCV dense cores.
  • Artificial intelligence methods to delineate vesicular membranes.

Related Experiment Videos

  • Quantitative assessment of vesicle shape (roundness) and spatial distribution (nearest-neighbor distance, Voronoi polygons).

    • Main Results:

    • A novel, rigorous quantitative morphological analysis of DCV spatial and regional properties was established.
    • Large vesicles were not found to be less round or possess larger free space, contrary to expectations for fusion.
    • Homotypic fusion was determined to be not prominent in PC-12 cells, irrespective of chronic treatment or enhanced release conditions.

    Conclusions:

    • The developed quantitative morphological analysis provides a robust method for studying DCV properties.
    • Homotypic fusion is not a major mechanism for DCV size regulation or dynamics in PC-12 cells.
    • Chronic treatment enhancing release does not induce significant homotypic fusion in these cells.